Essential Biochemistry - Enzymes (Section 5 - No. 25)
The primary mode of action for noncompetitive inhibitors involves:
Binding to the active site.
Altering substrate affinity.
Binding to a site other than the active site, affecting Vmax.
Reducing the substrate concentration.
Explication
Noncompetitive inhibitors bind at a site different from the active site, thereby affecting Vmax.